Outbreaks of Influenza of variable extent and severity occur nearly every year, resulting in significant morbidity rates in the general population and in increased mortality rates among high- patients. Apart from the annual seasonal epidemics, influenza presents sporadic and unpredictable global pandemic outbreaks as well. Pandemic influenza occurs every 10–50 years and is characterised by the introduction of a new influenza A virus strain that is antigenically very different from previously circulating strains; the lack of pre-existing immunity leads to increased mortality.
Therefore, in order to rapidly detect the presence of Influenza A(H1N1) aiming to an efficient and proactive management, a detection has been developed dedicated to this virus. More specifically, for Influenza A (H1N1) a LAMP-based portable quantitative real-time PCR (qPCR) has been developed, which can be easily used for samples' analysis. This device employs the technology of droplet microfluidics (DMF), its size is as small as a smartphone, and thus ideal for a POC device. Moreover, it can be connected to a PC or laptop via a USB port.
The complete of the detection of Influenza A (H1N1) is comprised by the development and validation of novel biomarkers targeting specifically the aforementioned virus strain, incorporated into a validated protocol which includes sample preparation and analysis. The qPCR DMF device is capable to detect the presence of Influenza A (H1N1) with robustness and accuracy, while due to the nature of microfluidics technology, it reduces the sample-to-result time.
A graphical representation of the implementation process is depicted in the illustrations.
Supported Use Cases
On-site detection of Influenza A (H1N1)
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